MCF7/182R-6isafulvestrant-resistantMCF7breastcancercellline.TheparentalMCF7/S0.5linewastreatedlong-termwith100nMfulvestrantandthesurvivingclonewasexpandedasaresistantcellline.Tomaintainahighlevelofresistance,thecellsareculturedinthepresenceof100nMfulvestrant.
Conditionofsale:theenduserisnotpermittedtotransferorre-freezethecells.
Donorgender | Female |
Donorageatsampling | 69yrs |
Geneticmodification | None |
Size | 1millioncells |
Growthproperties | Adherent |
Diseaseinformation | Malignantadenocarcinomaderivedfrompleural effusion |
Generalnotes | Expressestheestrogenreceptor(ER);resistanttofulvestrantat100nM |
Shippingconditions | Dryice |
Storageconditions | Liquidnitrogen |
Usagenotes | CultureinDMEM/F12medium(nophenolred)supplementedwith1%fetalbovineserum+2.5mMGlutaMAX+6ng/mLinsulin.Additionof100nMfulvestrantisrequiredtomaintainahighlevelofresistance |
Conditionofsale | Theenduserisnotpermittedtotransferorre-freezethecells |