MCF7/TAMR-4andMCF7/TAMR-8aretamoxifen-resistantMCF7breastcancercelllines.TheparentalMCF7/S0.5linewastreatedwith1μMtamoxifenfor28dayswhichkilledthemajorityofthecells.Thesurvivingcellswereclonallyexpandedassublinesintheabsenceoftamoxifenfor19passagesandwerethentreatedcontinuouslywith1μMtamoxifen.TheresistantcellscontinuedtoproliferateandapproachedagrowthratethatwascomparabletotheparentalMCF7/S0.5line.
Conditionofsale:theenduserisnotpermittedtotransferorre-freezethecells.
| Donorgender | Female |
| Donorageatsampling | 69yrs |
| Geneticmodification | None |
| Size | 1millioncells |
| Growthproperties | Adherent |
| Diseaseinformation | Malignantadenocarcinomaderivedfrompleural effusion |
| Generalnotes | Expressestheestrogenreceptor(ER);resistanttotamoxifenat1μM |
| Shippingconditions | Dryice |
| Storageconditions | Liquidnitrogen |
| Usagenotes | CultureinDMEM/F12medium(nophenolred)supplementedwith1%fetalbovineserum+2.5mMGlutaMAX+6ng/mLinsulin.Additionof1μMtamoxifenisrequiredtomaintainahighlevelofresistance |
| Conditionofsale | Theenduserisnotpermittedtotransferorre-freezethecells |



