MCF7/S0.5wasadaptedtogrowinlow-serumculturemediumtoreduceestrogenexposurefromfetalbovineserum.Thispermitsthecellstobeusedforexperimentsstudyinghormonesignalingandtheeffectsofdrugsthattargethormonereceptors.Thislinewasusedastheparentallinefromwhichthetamoxifen-resistantandfulvestrant-resistantMCF7celllinesweregenerated.Therefore,drugsensitiveandresistantmatchedcelllinesareavailable.
Conditionofsale:theenduserisnotpermittedtotransferorre-freezethecells.
Donorgender | Female |
Donorageatsampling | 69yrs |
Geneticmodification | None |
Size | 1millioncells |
Growthproperties | Adherent |
Diseaseinformation | Malignantadenocarcinomaderivedfrompleural effusion |
Generalnotes | Expressestheestrogenreceptor(ER) |
Shippingconditions | Dryice |
Storageconditions | Liquidnitrogen |
Usagenotes | CultureinDMEM/F12medium(nophenolred)supplementedwith1%fetalbovineserum+2.5mMGlutaMAX+6ng/mLinsulin |
Conditionofsale | Theenduserisnotpermittedtotransferorre-freezethecells |