MCF7/S0.5wasadaptedtogrowinlow-serumculturemediumtoreduceestrogenexposurefromfetalbovineserum.Thispermitsthecellstobeusedforexperimentsstudyinghormonesignalingandtheeffectsofdrugsthattargethormonereceptors.Thislinewasusedastheparentallinefromwhichthetamoxifen-resistantandfulvestrant-resistantMCF7celllinesweregenerated.Therefore,drugsensitiveandresistantmatchedcelllinesareavailable.
Conditionofsale:theenduserisnotpermittedtotransferorre-freezethecells.
| Donorgender | Female |
| Donorageatsampling | 69yrs |
| Geneticmodification | None |
| Size | 1millioncells |
| Growthproperties | Adherent |
| Diseaseinformation | Malignantadenocarcinomaderivedfrompleural effusion |
| Generalnotes | Expressestheestrogenreceptor(ER) |
| Shippingconditions | Dryice |
| Storageconditions | Liquidnitrogen |
| Usagenotes | CultureinDMEM/F12medium(nophenolred)supplementedwith1%fetalbovineserum+2.5mMGlutaMAX+6ng/mLinsulin |
| Conditionofsale | Theenduserisnotpermittedtotransferorre-freezethecells |



