AxolHumaniPSC-DerivedSensoryNeuronProgenitorsderivedfromahealthymaledonor.Axol"sinducedpluripotentstemcells(iPSCs)weredifferentiatedtosensoryneuronprogenitorsusingsmallmoleculeinhibitors.Forendpointassaysonplastic,SureBond-XFshouldbeusedforcoatingcellculturesurfaces.Forendpointassaysonglass,SureBond+ReadySetcoatingreagentshouldbeused.Afterthawingandplating,theprogenitorsneedtobegrowtharrestedandthenshouldbematuredforapproximately6weekspriortoendpointassays.TheexpressionofNav1.7shouldoccurataround4-5weeksofmaturationandNav1.8isexpressedafterapproximately6-8weeksofmaturationinSensoryNeuronMaintenanceMedium.
Startingmaterial | CordbloodCD34+cells |
Donorgender | Male |
Donorageatsampling | Newborn |
HLASEROtype | A29A68,B38(Bw4)B44(Bw4),Cw8Cw12 |
Karyotype | Normal |
Reprogrammingmethod | Episomalvector |
Inductionmethod | Monolayer&chemicallydefinedmedium |
Geneticmodification | None |
Size | 500,000cells |
Kitcomponents | 1vialofprogenitors(>500,000cells)and1bottleofNeuralPlating-XFMedium(30mL) |
Celltype | iPSC-derivedsensoryneuronprogenitors |
Growthproperties | Adherent |
Shippingconditions | Dryice |
Storageconditions | Liquidnitrogen |