HumaniPSC-DerivedNeuralStemCellsthathavebeengeneticallyeditedusingCRISPR-Cas9technologytointroducetheV337Mmutation(GTG>ATG) intheMAPTgene.ThislineisheterozygousfortheV337Mmutationwhere1allelehasthemutationandtheotheralleleiswildtype.Clickontheproductimagestoseethedataandfurtherdetails.
TheV337MmutationinMAPThasbeenimplicatedinfrontotemporaldementiaandmayhavearoleinAlzheimer"sdisease.ThemutationleadstoacceleratedaggregationofMAPT/tauproteinintofilamentstructures (Nacharajuetal.,1999)andmakestheMAPT/tauproteinmoresusceptIBLetohyperphosphorylation (Alonsoetal.,2004).
Startingmaterial | Dermalfibroblast |
Donorgender | Female |
Donorageatsampling | 64yrs |
Karyotype | Normal |
Reprogrammingmethod | Episomalvector |
Inductionmethod | Monolayer&chemicallydefinedmedium |
Geneticmodification | HeterozygousfortheMAPTV337Mmutation(GTG>ATG) |
Geneticmodification | Containsapuromycinresistancecassette(intronic) |
Size | ≥1.5millioncells |
Kitcomponents | 1vialofNeuralStemCells(≥1.5millioncells)and1bottleofNeuralPlating-XFMedium(30mL) |
Growthproperties | Adherent |
Shippingconditions | Dryice |
Storageconditions | Liquidnitrogen |