HumaniPSC-DerivedNeuralStemCellsthathavebeengeneticallyeditedusingCRISPR-Cas9technologytointroducetheR406Wmutation(CGG>TGG)intotheMAPTgene.ThislineisheterozygousfortheR406Wmutationwhereoneallelecontainsthemutationandtheotheralleleiswildtypeatthelocus.
TheR406WmutationinMAPThasbeenimplicatedinfamilialfrontotemporaldementiaandparkinsonism(Huttonetal.,1998;Behnametal.,2015)andhasbeenseeninpatientswithclinicalpresentationresemblingAlzheimer"sdisease(Rademakersetal.,2003;Lindquistetal.,2008).TheR406WmutationreducestheABIlityofMAPT/tautobindtomicrotubulesinvitro(Hongetal.,1998).
Startingmaterial | Dermalfibroblast |
Donorgender | Female |
Donorageatsampling | 64yrs |
Karyotype | Normal |
Reprogrammingmethod | Episomalvector |
Inductionmethod | Monolayer&chemicallydefinedmedium |
Geneticmodification | HeterozygousfortheMAPTR406Wmutation(CGG>TGG) |
Geneticmodification | Containsapuromycinresistancecassette(intronic) |
Size | ≥1.5millioncells |
Kitcomponents | 1vialofNeuralStemCells(≥1.5millioncells)and1bottleofNeuralPlating-XFMedium(30mL) |
Growthproperties | Adherent |
Shippingconditions | Dryice |
Storageconditions | Liquidnitrogen |