Description:
Next-generationneuralcellculturemediumrestrictedtoanimal-freecomponents.Ithasbeenoptimizedtosupportlowdensitycultureandlong-termfunctionalmaturation.
Xeno-FreeCellCultureMediumforoptimalmaintenanceof
humanneuralstemcellsandneuralcelltypes
Forlong-termneuronalcultures,Axol"s NeuralMaintenance-XFMedium providesanidealenvironmenttosupportaxonalelongationandsynapticactivity.NeuralStemCellsculturedinthismediummaintainthecapacitytodifferentiateintoneuronalandgliallineages.Themediumiscompletelydefinedensuringlot-to-lotconsistencyandreliableperformancebetweenexperiments.NeuralMaintenance-XFMediumcanmaintainhealthyneuronswhenculturedatlowdensity(10,000cells/cm2).
NeuralStemCells wereculturedona SureBond+ReadySet coatedculturevesselusing NeuralDifferentiation-XFMedium. 54hafterthawing,theNSCscellswereswitched to NeuralMaintenance-XFMedium.Theresultingneuronswerestainedfor TUJ1 expressionandcounterstainedusing DAPI. ImagetakenatDay14.
NeuralStemCells wereculturedona SureBond+ReadySet coatedculturevesselusing NeuralDifferentiation-XFMedium. 54hafterthawing,theneuralstemcellswereswitched to NeuralMaintenance-XFMedium. TheresultingneuronswerestainedusinganantibodytargetingMAP2.ThecellswerecounterstainedusingDAPI.ImagetakenatDay14.
NeuralStemCells werebrieflyexpandedinNeuralExpansion-XFMedium andpassagedontoa SureBond+ReadySet coatedculturevessel.Thecellsweremaintainedin NeuralMaintenance-XFMedium.Thecellswerecounterstainedusing DAPI.Neuralstemcellscanbevisualizedas Ki67-positivecells.NeuronsdifferentiatedfromtheneuralstemcellsareshownusingtheMarker TUJ1.ImagetakenatDay7.
ProductSpecification:
| Size | 500mL |
| Shippingconditions | Dryice |
| Storageconditions | -80°C |
| Xenofree | Yes |
Axol Bioscience公司位于英国剑桥，由Jonathan Milner博士(1998年创建Abcam)和Yichen Shi博士 (获剑桥大学神经科学和干细胞生物学博士学位)共同创立，为全球科研人员提供人iPSC来源的神经干细胞和神经元。Axol Bioscience利用前沿干细胞技术制备高品质的人类神经干细胞及神经元。大脑皮质神经元与多种神经系统疾病相关，包括阿尔茨海默病、自闭症、癫痫、外伤性脑损伤和中风等。尽管这些领域的研究技术不断发展，但仍缺乏高品质的体外细胞模型用于研究皮质神经生理学。Axol Bioscience利用其前沿技术提供iPSC来源的健康的及阿尔茨海默病相关的人类神经元。目前Axol可提供人类神经祖细胞、大脑皮质神经元、人类阿尔茨海默病神经祖细胞以及细胞培养试剂和转染试剂等。
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